not necessarily. with the exception of the PE's, the last 6 in the VIN sequence is computer generated and does not represent any particular 'order' of assembly.
for example, my VIN is CTXXX416 with a "Build Date" of 7/4/11. the sequential numbers following my VIN, 417, 418, 419 and 420 had build dates of 7/21/11, 7/21/11, 7/20/11 and 7/18/11 respectively. so the car with the next VIN after mine was built 17 days AFTER my car. the VIN before mine (CTXXX415) was built on 7/16/11.
while they were all made within a short time of each other, as you can see they were NOT made 'in order'.
even the PE's, while the VIN's are sequential, I've noticed that they were NOT necessarily made 'in order'.